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COT Nicotine Saliva Test Card (Forensic Use)

  • Non-invasive instant collection

  • 100 ng/mL cutoff concentration

  • Non-invasive

  • Simple sample collection using swab

  • Observable and no room needed

  • 2-years long shelf life

  • Results within 5 minutes


Test Item

Test per Box



COT 100ng/mL

COT    30ng/mL

25 tests

25 tests

* For label design and mold design, please be free to contact us.


The Cotinine Saliva Test (COT100) is a lateral flow chromatographic immunoassay for the detection of Cotinine in human saliva at the 100 ng/ml cut-off concentration.
This assay provides only a qualitative, preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result,
particularly when preliminary results are positive

How to use

Test must be in room temperature (50.0℉ ~86.0℉/10 ºC ~30 ºC).

  1. Open the sealed pouch by tearing along the notch. Remove the test device from the pouch.

  2. Holding a sample dropper vertically, add exactly four drops of the saliva specimen to the sample well.

  3. Read the result at 5 minutes. Do not read after 5 minutes.

Read the result

result reading

Positive (+)
One colored line appears in the control line region (C). No line appears in the test line region (T). This positive result indicates that the drug concentration exceeds the detectable level.

Negative (-)
Two lines appear. One colored line should be in the control line region (C), and another apparent colored line should be in the test line region (T). This negative result indicates that the drug concentration is below the detectable level.

Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test. If the problem persists, discontinue using the lot immediately and contact your local distributor with the lot number

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